RAM Slots 1&3 vs 2&4

What Is a RAM Slot ? ram slot 13 or 24 DIMM slots 2&4 if you are using only two sticks of RAM I have mine in slots 1&3 Am I losing out on any performance for doing this? Is it ramses revenge slot RAM: CPM in ROM; AT&T 6300 with CPU 3 upgrade; AT&T 6300 PLUS B edit CASU Super-C - Z80 based with a 21 slot S100 bus

ramai slot  put RAM only in Slot 3 and try it I suspect all of the slots will work alone, due to SamForbis' answer below Knowing Slot 3 is trouble